An Experimental Biospectroscopic Study on Seminal Plasma in Determination of Semen Quality for Evaluation of Male Infertility
نویسنده
چکیده
To investigate the correlations among seminal plasma Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations and semen parameters [1– 15]. 500 fertile and 1000 infertile males provided a standardized semen specimen. Total Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations were determined by Flame Atomic Absorption Spectroscopy (FAAS), Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR–FTIR), Energy–Dispersive X– Ray Spectroscopy (EDX), UV–Vis spectroscopy and FT–Raman spectroscopy. Semen analysis was performed according to the SigmaAldrich Corporation guidelines. The strong and positive correlation was found among seminal plasma Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations (p<0.001). Also, positive correlation was found among Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations and vitality and motility (p<0.0001). On the other hand, there is negative correlation among Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations and abnormal morphology (Figures 1–3). Therefore, the present data indicated that Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations could be useful factor in determination of semen quality.
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